天津科技大学生物工程学院欢迎您!
讲师

赵青,博士,天津科技大学生物工程学院讲师。


主讲课程:
本科生:《分子生物学》


教学成果:
天津科技大学第三届微生物艺术大赛优秀指导教师


从事研究的学科专业领域及主要研究方向:
1. 功能蛋白的筛选、制备与应用。
2. 工业酶的发掘、改造、制备与应用。


主持或参与科研项目:
1. 天津市食品加工过程控制与安全工程技术中心开放基金,《马兜铃酸纳米抗体替代物绿色免疫分析研究》,2018-2019,主持
2. 中国博士后科学基金面上项目,《电压门控质子通道Hv1胞内结构域的相互作用与功能研究》,2015-2017,主持
3. 天津市应用基础与前沿技术研究计划(一般项目),《质子通道Hv1质子传输机制及对肿瘤发展的作用机理研究》,2014-2017
4. 国家自然科学基金面上项目,《电压门控质子通道Hv1对肿瘤发生发展作用机制的研究》,2013-2016
5. 国家自然科学基金青年基金项目,《L-氨基酸脱氢酶手性选择性改造及机理研究》,2012-2015
6. 国家自然科学基金面上项目,《人源线粒体内膜转位因子Tim23和Tim50复合体结构与功能研究》,2010-2013
7. 中科院知识创新工程重要方向项目,《天冬氨酸的绿色生产工艺》,2009-2012
8. 国家自然科学基金主任基金,《人源线粒体内膜转位因子Tim23和Tim50复合体结构与功能研究》,2009-2010
9. 中国科学院知识创新工程项目,《新型酶催化剂的开发及其在有机合成中的应用研究》,2008-2011
10. 天津市应用基础与前沿技术研究计划(一般项目), 《人源电压门控质子质子通道Hv1的结构与功能研究》,2008-2011


代表性成果:
1. Tian Zhao, Zhipeng Li, Zhouliang Guo, Ali Wang, Zhenxing Liu, Qing Zhao, Yuyin Li, McKenzie EA, Aipo Diao*, Functional recombinant human Legumain protein expression in Pichia pastoris to enable screening for Legumain small molecule inhibitors, Protein Expr Purif., 2018, 150: 12-16.
2. Qing Zhao, Weiyan Zuo, Shangrong Zhang, Yongqiang Zhang, Chuanyong Li, Shu Jie Li*, Proton pump inhibitors have pH‑dependent effects on the thermostability of the  carboxyl‑terminal domain of voltage‑gated proton channel Hv1, Eur Biophys J, 2018, 47(3):237-247. doi: 10.1007/s00249-017-1253-3.
3. Xudong Wang, Wang Xi, Qing Zhao, Shangrong Zhang , Jiwei Qin, Jili Lv, Yongzhe Che, Weiyan Zuo, Shu Jie Li*, The Voltage-gated Proton Channel Hv1 Is Required for Insulin Secretion in Pancreatic β Cells, BioRxiv, 2017, doi: https://doi.org/10.1101/097816
4. Qing Zhao1, Xudong Wang1, Wang Xi, Shangrong Zhang, Jiwei Qin, Jili Lv, Yongzhe Che, Weiyan Zuo, Shu Jie Li*, Deficiency of Voltage-gated Proton Channel Hv1 Leads to hypoinsulinaemia, hyperglycemia and glucose intolerance in mice, BioRxiv, 2017, doi: http://dx.doi.org/10.1101/097816.
5. Qing Zhao, Yongzhe Che, Qiang Li, Shangrong Zhang, Ying-Tang Gao, Yifan Wang, Xudong Wang, Wang Xi, Weiyan Zuo, Shu Jie Li*, The voltage-gated proton channel Hv1 is expressed in pancreatic islet β-cells and regulates insulin secretion, Biochem. Biophys. Res. Commun., 2015, doi: 10.1016/j.bbrc.2015.11.027.
6. Qing Zhao, Chuanyong Li, Shu Jie Li#, The pH-sensitive structure of the C-terminal domain of voltage-gated proton channel and the thermodynamic characteristics of Zn2+ binding to this domain, Biochem. Biophys. Res. Commun., 2015, doi: 10.1016/j.bbrc.2014.11.060.
7. Qing Zhao, Yongqiang Zhang, Shu Jie Li*, Interaction of dievalent ions with human voltage-gated p,roton channel Hv1, Biometals, 2014, doi: 10.1007/s10534-014-9751-6.
8. Dalong Zhang, Rui Zhang, Jie Zhang, Liying Chen, Chunxia Zhao, Wenyue Dong, Qing Zhao, Qiaqing Wu, Dunming Zhu*, Engineering a hydroxysteroid dehydrogenase to improve its soluble expreesion for the asymmetric reduction of cortistone to 11 beta-hydrocortisone, Appl. Mocrobiol. Biotechnol., 2014, doi: 10.1007/s00253-014-5967-1.

9. Xiangtao Liu, Qing Zhao, Jie Ren, Wenyue Dong, Qiaqing Wu∗, Dunming Zhu*, N-terminal truncation of a maleate cis–trans isomerase from Rhodococcus jostii RHA1 results in a highly active enzyme for the biocatalytic production of fumaric acid, J. Mol. Catal. B: Enzym., 2013, doi: 10.1016/j.molcatb.2013.04.002
10. Yongqiang Zhang, Honghua Deng, Qing Zhao, Shu Jie Li*, Interaction of Presequence with human translocase of inner membrane of mitochondria Tim23, Biochem. Biophys. Res. Commun., 2013, doi: 10.1016/j.bbrc.2013.06.070.
11. Yongqiang Zhang, Honghua Deng, Qing Zhao, Shu Jie Li*, Interaction of Presequence with Human translocase of the Inner Membrane of Mitochondria Tim50, J. Phys. Chem. B, 2012, doi: 10.1021/jp2108279.
12. Yongqiang Zhang, Yun Xu, Qing Zhao, Zhina Ji, Honghua Deng, Shu Jie Li*, The structural characteristics of human preprotein translocase of the inner mitochondrial membrane Tim23: implications for its physiological activities, Protein Expr. Purif., 2012, doi: 10.1016/j.pep.2012.01.009.
13. Yongqiang Zhang, Yun Xu, Qing Zhao, Zhina Ji, Qiang Li, Shu Jie Li*, Expression and structural characterization of human translocase of inner membrane of mitochondria Tim50, Protein Expr. Purif., 2011, doi: 10.1016/j.pep.2011.06.012.
14. Yifan Wang, Shu Jie Li*, Juncheng Pan, Yongzhe Che, Jian Yin, Qing Zhao, Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis, Biochem. Biophys. Res. Commun., 2011, doi: 10.1016/j.bbrc.2011.07.102.
15. Shu Jie Li *, Qing Zhao, Qiangjun Zhou, Hideaki Unno, Yujia Zhai, and Fei Sun, The role and structure of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1, J. Biol. Chem., 2010, doi: 10.1074/jbc.M109.040360. 
16. Shu Jie Li*, Qing Zhao, Qiangjun Zhou and Yujia Zhai, Expression, purification, crystallization and preliminary crystallographic study of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1, Acta Crystallogr. 2009, doi: 10.1107/S1744309109003777.
17. 李树杰, 王一凡,王汉中,赵青, 潘君成。一种以电压门控质子通道Hv1作为乳腺癌治疗的靶向位点的基因药物, 专利号:201010297433,2010,已授权。
18. 朱敦明,吴洽庆,李正华,赵青,任杰,董文玥,刘祥涛。一种新的顺丁烯二酸异构酶,专利号:201110026297.5, 2011,已授权。

联系方式
办公地点:天津经济技术开发区第十三大街29号天津科技大学生物工程学院2-107
邮政编码:300457
办公电话:022-60602948
传 真:022-60602298
Email:zhao_qing@tust.edu.cn



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