天津科技大学生物工程学院欢迎您!
教授


秦慧民,博士,天津科技大学生物工程学院教授,博士生导师。中国微生物学会会员。

学习经历:

2010.4-2013.3 东京大学应用生命化学专业 博士

2004.9-2008.7 华南理工大学制糖工程专业 硕士

2000.9-2004.7 山东农业大学食品科学与工程专业 本科

工作经历:

2017/12-至今 天津科技大学, 教授; 主要研究方向: 发酵工程;酶工程

2015/6-2017/11 天津科技大学, 讲师; 主要研究方向: 发酵工程;酶工程

2013/4-2015/5 东京大学应用生命化学专业 特任助教

2008/10-2009/8 东北师范大学中国赴日本国留学生预备学校 日语学习

主讲课程:

本科生:微生物学实验,微生物遗传学实验

研究生:结构生物学

留学生:Microbial Genetics and Breeding Experiment; Introduction of Biology & Pharmacy; Protein Structure Simulation and Design

科研领域及方向

[1] 功能糖酶制剂研究与开发;[2]酶的结构与功能解析;[3] 酶的理性设计与定向进化

科研情况

[1] 国家自然科学基金面上项目,维生素D3前体生物合成关键酶胆固醇7-脱氢酶系的构效研究,2018.1-2021.12,在研,主持项目

[2] 天津市应用基础与前沿技术研究计划,稀少糖转化酶D-阿洛酮糖3-差向异构酶的高通量筛选与定向改造,2019.11-2022.10, 在研,主持项目

[3] 天津市自然科学基金青年项目,海藻酸多糖降解酶的结构生物学与功能研究,2016.4-2019.3 结题,主持项目

[4] 天津市引进人才科研启动项目,工业化应用相关酶的结构生物学基础研究,2015.9-2017.12, 结题,主持项目

主要学术成果

近期代表作

2020年

1) Wang, F., Zhu, M., Song, Z., Zhu, Z., Lu, F.,* Qin, H.-M.* Reshaping the binding pocket of lysine hydroxylase for enhanced activity. ACS Catal. 2020, 10: 13946-13956

2) Zhu, Z., Gao, X., Song, Z., Li, C., Lu, F.*, Tanokura, M.*, Qin, H.-M.* Development of engineered ferredoxin reductase systems for the efficient hydroxylation of steroidal substrates. ACS Sustain. Chem. Eng. 2020, 8: 16720-16730;

3) Mao, S., Liu, X., Gao, X., Zhu, Z., Sun, D., Lu, F.*, Qin, H.-M.* Design of an efficient whole-cell biocatalyst for the production of hydroxyarginine based on a multi-enzyme cascade. Bioresource Technol. 2020, 318, 124261

4) Li, C., Wu, M., Gao, X., Zhu, Z., Li, Y., Lu, F.*, Qin, H.-M.* Efficient biosynthesis of 2'-fucosyllactose using an in vitro multienzyme cascade. J. Agric. Food Chem. 2020, 68: 10763-10771

5) Qin, H.-M., Gao, D., Zhu, M., Li, C., Zhu, Z., Wang, H., Liu, W.*, Tanokura, M.*, Lu, F.* Biochemical characterization and structural analysis of ulvan lyase from marine Alteromonas sp. reveals the basis for its salt tolerance. Int. J. Biol. Macromol. 2020,147, 1309-1317

6) Mao, S., Song, Z., Wu, M., Wang, X., Lu, F.*, Qin, H.-M.* Expression, purification, refolding and characterization of a neverland protein from Caenorhabditis elegans. Front. Bioeng. Biotechnol. 2020, doi: 10.3389/fbioe.2020.593041

7) Mao, S., Cheng, X., Zhu, Z., Chen, Y., Li, C., Zhu, M., Liu, X., Lu, F.*, Qin, H.-M.* Engineering a thermostable version of D-allulose 3-epimerase from Rhodopirellula baltica via site-directed mutagenesis based on B-factors analysis. Enzyme Microb. Technol. 2020, 132: 109441

2019年

1) Sun, D., Gao, D., Liu, X., Zhu, M., Li, C., Chen, Y., Zhu, Z., Lu, F.*, Qin, H.-M.* Redesign and engineering of a dioxygenase targeting biocatalytic synthesis of 5-hydroxyl leucine. Catal. Sci & Technol. 2019, 9: 1825-1834

2) Sun, D., Liu, X., Zhu, M., Chen, Y., Li, C., Cheng, X., Zhu, Z., Lu, F.*, Qin, H.-M.* Efficient biosynthesis of high-value succinic acid and 5-hydroxyleucine using a multienzyme cascade and whole-cell catalysis. J. Agric. Food Chem. 2019,67, 12502-12510

3) Zhu, Z., Gao, D., Li, C., Chen, Y., Zhu, M., Liu, X., Tanokura, M.*, Qin, H.-M.*, Lu, F. Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose. Microb. Cell Fact. 2019, 18:59

4) Mao, S., Guo, Q., Xu, P., Gao, D., Cheng, X., Zhu, M., Sun, D., Zhu, Z., Lu F.,* Qin, H.-M.* Biochemical and structural characterization of 3-ketosteroid-∆1-dehydrogenase, a candidate enzyme for efficient bioconversion of steroids. J. Chem. Technol. Biotechnol. 2019, 94: 309-316

5) Zhu, Z., Li, C., Liu, X., Gao, D., Wang, X., Tanokura, M.*, Qin, H.-M.*, and Lu, F.* Biochemical characterization and biocatalytic application of a novel D-tagatose 3-epimerase from Sinorhizobium sp. RSC Adv. 2019, 9: 2919-2927

6) Mao, S., Liu, Y., Yang, J. Ma, X., Zeng, F., Zhang, Z., Wang, S., Han, H., Qin, H.-M.*, Lu, F.* Cloning, expression and characterization of a novel fructosyltransferase from Aspergillus niger and its application in the synthesis of fructooligosaccharides. RSC Adv. 2019, 9: 23856-23863

7) Zhu, Z., Li, C., Cheng, X., Chen, Y., Zhu, M., Liu, X., Mao, S., Qin, H.-M.*, Lu, F.* Soluble expression, purification and biochemical characterization of a C-7 cholesterol dehydrogenase from Drosophila melanogaster. Steroids 2019, 152, 108495

2018

1) Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Ojima, T., Tanokura, M. Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family. Chem. Commun. 2018, 54: 555-558

2) Mao, S., Wang, J.-W., Liu, F., Zhu, Z., Gao, D., Guo, Q., Xu, P., Ma, Z., Hou, Y., Cheng X., Sun, D., Lu, F.*, Qin, H.-M.* Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates. Microb. Cell Fact. 2018, 17:141

3) Mao, S., Li, K., Hou, Y., Liu, Y., Ji, S., Qin, H.-M.*, Lu, F.* Synergistic effects of components in deep eutectic solvents relieve toxicity and improve the performance of steroid biotransformation catalyzed by Arthrobacter simplex, J. Chem. Technol. Biotechnol. 2018, 93: 2729–2736

4) Qin, H.-M., Xu, P., Guo, Q., Cheng, X., Gao, D., Sun, D., Zhu, Z., Lu, F., Biochemical characterization of a novel ulvan lyase from Pseudoalteromonas sp. strain PLSV. RSC Adv. 2018, 8: 2610-2615

5) Mao, S., Liu, Y., Hou, Y., Ma, X., Yang, J., Han, H., Wu, J., Jia, L., Qin, H.*, and Lu, F.* Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892, RSC Adv. 2018, 8: 39897-39901

6) Sun, D., Gao, D., Xu, P., Guo, Q., Zhu, Z., Cheng, X., Bai, S., Qin, H.-M.*, Lu, F.* A novel L-leucine 5-hydroxylase from Nostoc piscinale unravels unexpected sulfoxidation activity toward L-methionine, Protein Expres. Purif. 2018, 149, 1-6

前期代表作

[1] Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Sawano, Y. Ojima, T., Tanokura, M. Structure and polymannuronate specificity in a eukaryotic member of the polysaccharide lyase family 14. J. Biol. Chem. 2017, 292, 2182-2190

[2] Qi, Z., Zhu, Z., Wang, J.-W., Li, S., Guo, Q., Xu, P., Lu, F. Qin, H.-M.* Biochemical analysis and the preliminary crystallographic characterization of D-tagatose 3-epimerase from Rhodobacter sphaeroides. Microb. Cell Fact. 2017,16: 193

[3] Qin, H.-M., Zhu, Z., Ma, Z., Xu, P., Guo, Q., Li, S., Wang, J.-W., Mao, S., Liu, F., Lu F. Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates. Sci. Rep. 2017, 7: 16375

[4] Qin, H.-M., Miyakawa, T., Inoue, A., Nakamura, A., Nishiyama, R., Ojima, T., Tanokura, M. Laminarinase from Flavobacterium sp. reveals the structural basis of thermostability and substrate specificity. Sci. Rep. 2017, 7, 11425

[5] Qin, H.-M., Xu, P., Guo, Q., Cheng, X., Gao, D., Sun, D., Zhu, Z., Lu, F., Biochemical characterization of a novel ulvan lyase from Pseudoalteromonas sp. strain PLSV. RSC Adv. 2018, 8: 2610-2615

[6] Li, Y., Wei, L., Zhu, Z., Li, S., Wang, J.-W., Xin, Q., Wang, H., Lu F., Qin, H.-M.* Rational design to change product specificities and thermostability of cyclodextrin glycosyltransferase from Paenibacillus sp. RSC Adv. 2017, 7, 13726-13732

[7] Qin, H.-M., Wang, J.-W., Guo, Q., Li, S., Xu, P., Zhu, Z., Lu F. Refolding of a novel cholesterol oxidase from Pimelobacter simplex reveals dehydrogenation activity. Protein Expres. Purif. 2017, 139, 1-7

[8] Qin, H.-M., Li, S., Zhang, Y.-F., Wang, J.-W., Li, J., Song, S., Lu, F., Li, Y. Multienzymatic cascade synthesis of fucosyloligosaccharides via a two-step fermentation strategy in Escherichia coli. Biotechnol. Lett. 2016, 38, 1747-1752

[9] Qin, H.-M., Imai, F. L., Miyakawa, T., Kataoka, M., Okai, M., Ohtsuka, J., Hou, F., Nagata, K., Shimizu, S. and Tanokura, M. L-allo-Threonine aldolase with H128Y/S292R mutation from Aeromonas jandaei DK-39 reveals the structural basis of change for substrate stereoselectivity. Acta Crystallogr. D, Biological Crystallography. 2014, 70, 1695-1703

[10] Qin, H.-M., Yamamura, A., Miyakawa, T., Kataoka, M., Nagai, T., Kitamura, N., Urano, N., Maruoka, S., Ohtsuka, J., Nagata, K., Shimizu, S. and Tanokura, M. Structure of conjugated polyketone reductase from Candida parapsilosis IFO 0708 reveals conformational changes for substrate recognition upon NADPH binding. Appl. Microbiol. Biot. 2014, 98, 243-249

[11] Qin, H.-M., Miyakawa, T., Nakamura, A., Hibi, M., Ogawa, J., and Tanokura, M. Structural optimization of SadA, an Fe(II)- and a-ketoglutarate-dependent dioxygenase targeting biocatalytic synthesis of N-succinyl-L-threo-3,4-dimethoxyphenylserine. Biochem. Bioph. Res. Co. 2014, 450, 1458-1461

[12] Nakamura, H.,* Xue, Y.-L.,* Miyakawa, T.,* Hou, F., Qin, H.-M., Fukui, K., Shi, X., Ito, E., Ito, S., Park, S.-H. Miyauchi, Y., Asano, A., Ueda, T., Tanokura, M., Asami. T. Molecular mechanism of strigolactone perception by DWARF14 in plant. Nature commun. 2013, 4, 2613

[13] Qin, H.-M., Miyakawa, T., Jia, M.-Z., Nakamura, A., Ohtsuka, J., Xue, Y.-L., Kawashima, T., Kasahara, T., Hibi, M., Ogawa, J. and Tanokura, M. Crystal structure of a novel N-substituted L-amino acid dioxygenase from Burkholderia ambifaria AMMD. PLOS ONE 2013, e63996

[14] Qin, H.-M., Yamamura, A., Miyakawa, T., Kataoka, M., Maruoka, S., Ohtsuka, J., Nagata, K., Shimizu, S. and Tanokura, M. Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH. Proteins 2013, 81,2059-2063

[15] Qin, H.-M., Miyakawa, T., Nakamura, A., Xue, Y.-L., Kawashima, T., Kasahara, T., Hibi, M., Ogawa, J. and Tanokura, M. Expression, purification, crystallization and preliminary X-ray analysis of a branched-chain amino acid dioxygenase from Burkholderia ambifaria AMMD. Acta Crystallogr. F 2012, 68, 1067-1069

获奖情况

2009-2013 日本政府(文部科学省)国费奖学金——大使馆推荐

2013年 东京大学农学生命科学研究科长奖励

联系方式

办公地点:天津经济技术开发区第十三大街29号天津科技大学生物工程学院

邮政编码:300457

办公电话:022-60602949


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