天津科技大学生物工程学院欢迎您!
教授




谢希贤理学博士,教授,博士生导师。入选国家“万人计划”科技创新领军人才,科技部中青年科技创新领军人才,现任代谢控制发酵技术国家地方联合工程实验室副主任,天津微生物学会理事,天津市食品学会理事。


教育科研背景

[1] 2012.32013.3     Rice University, USA                访问学者

[2] 2004.92007.7 厦门大学生命科学学院      理学博士

[3] 2001.92004.7 国家海洋局第三海洋研究所          理学硕士

[4] 1995.92000.7 中国科学技术大学生命科学学院   理学学士

主讲课程

[1] 本科生课程:代谢控制发酵

[2] 研究生课程:代谢调控


主要教学研究及成果情况

[1] 工业微生物学(第二版高等教育出版社,2021

[2] 氨基酸工艺学(第二版),中国轻工业出版社,2020

[3] 微生物遗传育种,高等教育出版社,2020

[4] 发酵食品,中国质检出版社,2013


科研领域及方向

[1] 代谢工程:氨基酸和核苷酸及衍生物代谢工程育种、代谢网络定量分析、氨基酸和核苷酸及衍生物产品开发和应用;

[2] 发酵工程:发酵过程优化放大,产品分离提取,节能减排和资源高效利用;

[3] 系统生物学:重要工业微生物生理代谢、基因组和蛋白质组等比较组学研究。


科研项目情况

[1] 国家重点研发计划:版本模式微生物底盘细胞系统集成与功能测试,2019-2024

[2] 宁夏重点研发计划:新型L-精氨酸高产工程菌的开发及清洁化工艺技术研究,2020-2022

[3] 天津市科技支撑计划:发酵法生产核苷类药物利巴韦林,2016-2019

[4] 国家自然科学基金:谷氨酸棒杆菌分支链氨基酸代谢网络比较分析及动态调控研究,2015-2018

[5] 国家863计划:有机酸生物合成途径构建与优化技术,2015-2017

[6] 国家自然科学基金:低温嘌呤核苷磷酸化酶在利巴韦林发酵中的应用基础研究,2012-2014

[7] 国家科技支撑计划:抗生素、维生素、氨基酸等大宗产品的微生物发酵及分离纯化技术,2008-2010

[8] 国家科技支撑计划:5万吨/年谷氨酸元素循环酸碱再生耦联技术与示范,2011-2013

[9] 发酵行业共性技术开发专项:甜菜碱等产酸促进剂对氨基酸发酵影响的研究,2010-2011

[10] 天津市科技支撑计划:利用黄色短杆菌高效生产L-缬氨酸,2008-2011

[11] 天津市教委基金:微生物发酵法生产利巴韦林的研究,2007-2010

[12] 企业技术开发:厌氧发酵法生产缬氨酸菌种构建,2020-2022

[13] 企业技术开发:谷氨酸衍生物技术开发,2019-2039

[14] 企业技术开发:生物发酵法生产丝氨酸技术,2018-2038

[15] 企业技术开发:高产L-色氨酸菌株委托开发2018-2019

[16] 企业技术开发:高产精氨酸和瓜氨酸发酵技术开发,2018-2020

[17] 企业技术开发:生物发酵法生产组氨酸技术2017-2027

[18] 企业技术开发:四氢嘧啶发酵生产技术的开发,2017-2018

[19] 企业技术开发:苏氨酸或色氨酸前体或衍生物开发,2015-2016

[20] 企业技术开发:氨基酸生产菌株高产抗逆机制发现与菌株选育,2014-2015

[21] 企业技术开发L-缬氨酸发酵生产技术的开发,2010-2011

[22] 企业技术开发:鸟苷发酵生产技术的开发,2008-2010

代表性论文

[1] Hao Y, Ma Q, Liu X, Fan X, Men J, Wu H, Jiang S, Tian D, Xiong B, Xie X*. High-yield production of L-valine in engineered Escherichia coli by a novel two-stage fermentation. Metab Eng. 2020. 62:198-206.  IF: 9.783

[2] Wu H, Li Y, Ma Q, Li Q, Jia Z, Yang B, Xu Q, Fan X, Zhang C, Chen N, Xie X*. Metabolic engineering of Escherichia coli for high-yield uridine production. Metab Eng, 2018, 49:248-256.  IF: 8.201

[3] Ning Y, Wu X, Zhang C, Xu Q, Chen N, Xie X*. Pathway construction and metabolic engineering for fermentative production of ectoine in Escherichia coli. Metab Eng, 2016, 36:10-18.  IF: 8.201

[4] Zhang C, Li Y, Ma J, Liu Y, He J, Li Y, Zhu F, Meng J, Zhan J, Li Z, Zhao L, Ma Q, Fan X, Xu Q, Xie X, Chen N*. High production of 4-hydroxyisoleucine in Corynebacterium glutamicum by multistep metabolic engineering. Metab Eng, 2018, 49:287-298.  IF: 8.201

[5] Wu H, Tian D, Fan X, Fan W, Zhang Y, Jiang S, Wen C, Ma Q, Chen N, Xie X*. Highly Efficient Production of l-Histidine from Glucose by Metabolically Engineered Escherichia coli. ACS Synth Biol. 2020. 9(7):1813-1822.  IF: 5.110

[6] Xiong B, Zhu Y, Tian D, Jiang S, Fan X, Ma Q, Wu H, Xie X*. Flux redistribution of central carbon metabolism for efficient production of L-tryptophan in Escherichia coli. Biotechnol Bioeng, 2021, 118(3), 1393-1404.  IF: 4.530

[7] Fan X, Wu H, Jia Z, Li G, Li Q, Chen N, Xie X*. Metabolic engineering of Bacillus subtilis for the co-production of uridine and acetoin. Appl Microbiol Biotechnol, 2018, 102(20):8753-8762.  IF: 3.340

[8] Zhang C, Qi J, Li Y, Fan X, Xu Q, Chen N, Xie X*. Production of α-ketobutyrate using engineered Escherichia coli via temperature shift. Biotechnol Bioeng, 2016, 113(9):2054-9.  IF: 4.126

[9] Li Y, Wei H, Wang T, Xu Q, Zhang C, Fan X, Ma Q, Chen N, Xie X*. Current status on metabolic engineering for the production of l-aspartate family amino acids and derivatives. Bioresour Technol, 2017, 245:1588-1602. IF: 5.651

[10] Ma Y, Chen Q, Cui Y, Du L, Shi T, Xu Q, Ma Q, Xie X, Chen N. Comparative genomic and genetic functional analysis of industrial L-leucine- and L-valine-producing Corynebacterium glutamicum strains. J Microbiol Biotechnol, 2018, doi: 10.4014/jmn.1805.05013.  IF: 1.650

[11] Fan X, Wu H, Li G, Yuan H, Zhang H, Li Y, Xie X*, Chen N. Improvement of uridine production of Bacillus subtilis by atmospheric and room temperature plasma mutagenesis and high-throughput screening. PLoS One, 2017, 12(5):e0176545.  IF: 3.234

[12] Ma Q, Zhang Q, Xu Q, Zhang C, Li Y, Fan X, Xie X*, Chen N. Systems metabolic engineering strategies for the production of amino acids. Synth Syst Biotechnol, 2017, 2: 87-96.

[13] Gui Y, Ma Y, Xu Q, Zhang C, Xie X*, Chen N. Complete genome sequence of Corynebacterium glutamicum CP, a Chinese l-leucine producing strain. J Biotechnol, 2016, 220:64-65.  IF: 2.871

[14] Li Y, Sun L, Feng J, Wu R, Xu Q, Zhang C, Chen N, Xie X*. Efficient production of α-ketoglutarate in the gdh deleted Corynebacterium glutamicum by novel double-phase pH and biotin control strategy. Bioprocess Biosys Eng, 2016, 39(6):967-76.  IF: 1.997

[15] Xie X, Liang Y, Liu H, Liu Y, Xu Q, Zhang C, Chen N*. Modification of glycolysis and its effect on the production of L-threonine in Escherichia coli. J Ind Microbiol Biotechnol, 2014, 41:1007-1015.  IF: 2.439

[16] Wang J, Wen B, Wang Jian, Xu Q, Zhang C, Chen N, Xie X*. Enhancing l-isoleucine production by thrABC overexpression combined with alaT deletion in Corynebacterium glutamicum. Appl Biochem Biotech, 2013, 171(1):20-30. IF: 1.893

[17] Guo X, Wang J, Xie X, Xu Q, Zhang C, Chen N*. Enhancing the supply of oxaloacetate for L-glutamate production by pyc overexpression in different Corynebacterium glutamicum. Biotechnol Lett. 2013, 35(6):943-950. IF: 1.736

[18] Liang J, Zhang D, Guo X, Xu Q, Xie X, Zhang C, Bai G, Xiao X, Chen N*. At-line near-infrared spectroscopy for monitoring concentrations in temperature-triggered glutamate fermentation. Bioprocess Biosyst Eng. 2013, 36(12):1879-1887.  IF: 1.823

[19] Fang H, Xie X, Xu Q, Zhang C, Chen N*. Enhancement of cytidine production by coexpression of gnd, zwf, and prs genes in recombinant Escherichia coli CYT15. Biotechnol Lett. 2013, 35(2):245-251. IF: 1.736

[20] Liu Q, Cheng Y, Xie X, Xu Q, Chen N*. Modification of tryptophan transport system and its impact on production of L-tryptophan in Escherichia coli. Bioresour Technol. 2012, 114:549-554.  IF: 4.75

[21] Xie X, Huo W, Xia J, Xu Q, Chen N*. Structure-activity relationship of a cold-adapted purine nucleoside phosphorylase by site-directed mutagenesis. Enzyme Microb Technol. 2012, 51(1):59-65. IF: 2.592

[22] Xie X, Xu L, Shi J, Xu Q, Chen N*. Effect of transport proteins on L-isoleucine production with the L-isoleucine-producing strain Corynebacterium glutamicum YILW. J Ind Microbiol Biotechnol. 2012, 39(10): 1549-1556.  IF: 2.321

[23] Shen T, Liu Q, Xie X, Xu Q, Chen N*. Improved production of tryptophan in genetically engineered Escherichia coli with TktA and PpsA overexpression. J Biomed Biotechnol. 2012, 2012:605219.  IF: 2.88

[24] Xie X, Xia J, Xu Q, He K, Lu L, Chen N*. Low-molecular-mass homotrimer purine nucleotide phosphorylase: characterization and application in enzymatic synthesis of nucleoside antiviral drugs. Biotechnol Lett. 2011, 33(6):1107-1112. IF: 1.683

[25] Xie X, Wang G, Xia J, Chen N. Characterization of a recombinant cold-adapted purine nucleoside phosphorylase and its application in ribavirin bioconversion. World J Microbiol Biotechnol. 2011, 27:1175-1181.  IF: 1.532


授权发明专利

[1] Hydroxy-and dicarboxylic-fat synthesis by microbes, US 2015/0225753 A1.

[2] Xylose-induced genetically engineered bacteria used for producing ectoine and use thereof, PCT/CN2017/088284.

[3] Genetically engineered bacteria with high-yield uridine and its construction method and use, PCT/CN2018/072020.

[4] 一株高产L-缬氨酸的基因工程菌及其构建方法与应用,ZL201910791280.5

[5] 大肠杆菌基因工程菌及其发酵同步生产L-色氨酸与L-缬氨酸的用途,ZL2018108407194

[6] 生产L-精氨酸的基因工程菌及其构建方法与应用,ZL201911211097X

[7] 高产L-亮氨酸的相关基因及工程菌构建方法与应用,ZL2018112561433

[8] 一种生产L-组氨酸的基因工程菌及其应用,ZL202010151245X

[9] 一种L-脯氨酸-4-羟化酶及其基因工程菌、构建方法与应用,ZL2018106949649

[10] 一株高产L-缬氨酸的基因工程菌及发酵生产L-缬氨酸方法ZL2019110433187

[11] 一株大肠杆菌基因工程菌及利用其生产L-苏氨酸的方法,ZL2017100128916

[12] 一种ɑ-酮基丁酸的发酵生产工艺,ZL2015105795531

[13] 大肠杆菌基因工程菌的构建及其生产L-色氨酸的用途,ZL2018106976792

[14] 一种谷氨酸棒状杆菌与应用,ZL2016106474301

[15] 一种产谷氨酸的温敏型重组谷氨酸棒状杆菌及其应用,ZL2016101393026

[16] 一种增强zwf基因启动子表达强度的方法,ZL2017100128598

[17] 一种耐酸性苏氨酸生产菌及其构建方法与应用,ZL2015105790059

[18] 一株谷氨酸棒状杆菌及其高产异亮氨酸的方法,ZL2016102707711

[19] 高产嘧啶核苷的菌株及其氨甲酰磷酸合成酶调节位点,ZL2015110350701

[20] 一株高产嘧啶核苷的菌株及其氨甲酰磷酸合成酶调节位点,ZL2015110351418

[21] 利用木糖诱导产生四氢嘧啶的基因工程菌及其应用,ZL2017100128456

[22] 高产尿苷的基因工程菌及其构建方法与应用,ZL2018100209443

[23] 一种联产尿苷和乙偶姻的发酵生产方法,ZL201610137185X

[24] 过表达异源谷氨酰胺合成酶的基因工程菌及构建方法,ZL201711031742.0

[25] 一种产生四氢嘧啶的基因工程菌及其构建方法与应用,ZL201510410080.2

[26] 一种色氨酸连续发酵分离装置,ZL201620734548.3


获奖情况

[1] 羟基化氨基酸生物合成关键技术及产业化,河南省,科技进步奖,三等奖,2018

[2] 玉米原料高效清洁生产谷氨酸关键技术与产业化,黑龙江省,科技进步奖,一等奖,2017

[3] 甜菜碱提高氨基酸生产关键技术及产业化,中国轻工业联合会,科技进步奖,一等奖,2017

[4] 谷氨酸温度敏感突变株代谢调控关键技术及产业化,中国商业联合会,科技进步奖,特等奖,2017

[5] 伦世仪教育基金杰出青年学者,伦世仪教育基金理事会,2016

[6] 芳香族氨基酸及衍生物关键技术研究与产业化,中国轻工业联合会,科技进步奖,一等奖,2015

[7] 谷氨酸清洁生产和废水综合利用关键技术研究与应用,中国轻工业联合会,科技进步奖,二等奖,2015

[8] 分支链氨基酸代谢调控技术及产业化,天津市,科技进步奖,二等奖,2013


联系方式

办公地点:天津经济技术开发区第十三大街29号,天津科技大学生物工程学院

邮政编码300457

办公电话:022-60601251


Email: xixianxie@tust.edu.cn